Soluble solids in grape juice, reconstituted grape juice, concentrated grape juice, and grape nectar
RESOLUTION OIV-OENO 662F-2023
SOLUBLE SOLIDS IN GRAPE JUICE, RECONSTITUTED GRAPE JUICE, CONCENTRATED GRAPE JUICE, AND GRAPE NECTAR
Type I method
THE GENERAL ASSEMBLY,
IN VIEW of Article 2, paragraph iv of the Agreement of 3 April 2001 establishing the International Organisation of Vine and Wine,
AT THE PROPOSAL of the "Methods of Analysis" Sub-Commission,
CONSIDERING that for grape juice, reconstituted grape juice, concentrated grape juice, and grape nectar is proposed the application of the Method OIV-MA-AS2-02 the Compendium of International Methods of Analysis of Wines and Musts,
DECIDES to add the following method:
SOLUBLE SOLIDS IN GRAPE JUICE, RECONSTITUTED GRAPE JUICE, CONCENTRATED GRAPE JUICE, AND GRAPE NECTAR
1. Scope
This document describes the method used for determining soluble solids in grape juice, reconstituted grape juice, concentrated grape juice, and grape nectar, at the indicated concentrations ranging from 10.0 °Bx to 69.2 °Bx.
2. Principle
The soluble solids content of a sample is estimated from its refractive index at 20 °C, expressed either as an absolute value or as a percentage by mass of saccharose (degrees Brix), the value is set out on the Table II to obtain the sugar content in grams per litre and in grams per kilogram for grape juice, reconstituted grape juice, concentrated grape juice or grape nectar.
3. Apparatus
3.1. Abbe refractometer
The refractometer used must be fitted with a scale giving:
- either percentage by mass of saccharose to 0.1 %;
- or refractive indices to four decimal places.
The refractometer must be equipped with a thermometer having a scale extending at least from + 15 °C to + 25 °C and with a system for circulating water that will enable measurements to be made at a temperature of 20 ± 5 °C. The operating instructions for this instrument must be strictly adhered to, particularly with regard to calibration and the light source.
Note: An electronic refractometer can also be used. In this case, a correction is not necessary for devices that automatically take temperature into account.
3.2. Analytical balance verified and calibrated with minimum precision of 0.01 g.
4. Preparation of the sample
Pass the sample through a filter paper or dry gauze folded into four and, after discarding the first drops of the filtrate, carry out the determination on the filtered product.
In the case of concentrated grape juice, depending on its concentration, use the sample directly or prepare a dilution weighing 200 g of concentrated grape juice and adding purified water for laboratories (EN ISO 3696) up to 500 g (F = 2.5).
5. Procedure
Bring the sample to a temperature close to 20 °C. Place a small test sample on the lower prism of the refractometer, taking care (because the prisms are pressed firmly against each other) that this test sample covers the glass surface uniformly. Carry out the measurement in accordance with the operating instructions of the instrument manufacturer used. Read the percentage by mass of sucrose to within one decimal place or read the refractive index to four decimal places. Carry out at least two determinations on the same prepared sample. Note the temperature in t °C.
6. Calculation
6.1. Temperature correction
- Instruments graduated in percentage by mass of sucrose: use Table I to obtain the temperature correction.
Table I - Correction to be made in the case where the percentage by mass of saccharose was determined at a temperature different by 20°C.
 |
It is preferable that the variations in temperature in relation to 20 °C do not exceed 5 °C.
- Instruments graduated in refractive index: find the refractive index measured (2nd column) in Table II to obtain the corresponding value of the percentage by mass of sucrose (1st column). This value is corrected for temperature and expressed as a concentration at 20°C by means of Table I.
TABLE II - Table giving the sugar content of musts and concentrated musts in grammes per litre and in grammes per kilogramme, determined using a graduated refractometer, either in percentage by mass of saccharose at 20 °C or refractive index at 20 °C. The mass density at 20 °C is also given.
|
Saccharose (%(m/m) |
Refractive Index at 20 °C |
Mass Density at 20 °C |
Sugars (g/L) |
Sugars (g/kg) |
ABV (% v/v) at 20 °C |
|
10.0 |
1.34782 |
1.0391 |
82.2 |
79.1 |
4.89 |
|
10.1 |
1.34798 |
1.0395 |
83.3 |
80.1 |
4.95 |
|
10.2 |
1.34813 |
1.0399 |
84.3 |
81.1 |
5.01 |
|
10.3 |
1.34829 |
1.0403 |
85.4 |
82.1 |
5.08 |
|
10.4 |
1.34844 |
1.0407 |
86.5 |
83.1 |
5.14 |
|
10.5 |
1.34860 |
1.0411 |
87.5 |
84.1 |
5.20 |
|
10.6 |
1.34875 |
1.0415 |
88.6 |
85.0 |
5.27 |
|
10.7 |
1.34891 |
1.0419 |
89.6 |
86.0 |
5.32 |
|
10.8 |
1.34906 |
1.0423 |
90.7 |
87.0 |
5.39 |
|
10.9 |
1.34922 |
1.0427 |
91.8 |
88.0 |
5.46 |
|
11.0 |
1.34937 |
1.0431 |
92.8 |
89.0 |
5.52 |
|
11.1 |
1.34953 |
1.0436 |
93.9 |
90.0 |
5.58 |
|
11.2 |
1.34968 |
1.0440 |
95.0 |
91.0 |
5.65 |
|
11.3 |
1.34984 |
1.0444 |
96.0 |
92.0 |
5.71 |
|
11.4 |
1.34999 |
1.0448 |
97.1 |
92.9 |
5.77 |
|
11.5 |
1.35015 |
1.0452 |
98.2 |
93.9 |
5.84 |
|
11.6 |
1.35031 |
1.0456 |
99.3 |
94.9 |
5.90 |
|
11.7 |
1.35046 |
1.0460 |
100.3 |
95.9 |
5.96 |
|
11.8 |
1.35062 |
1.0464 |
101.4 |
96.9 |
6.03 |
|
11.9 |
1.35077 |
1.0468 |
102.5 |
97.9 |
6.09 |
|
12.0 |
1.35093 |
1.0472 |
103.5 |
98.9 |
6.15 |
|
12.1 |
1.35109 |
1.0477 |
104.6 |
99.9 |
6.22 |
|
12.2 |
1.35124 |
1.0481 |
105.7 |
100.8 |
6.28 |
|
12.3 |
1.35140 |
1.0485 |
106.8 |
101.8 |
6.35 |
|
12.4 |
1.35156 |
1.0489 |
107.8 |
102.8 |
6.41 |
|
12.5 |
1.35171 |
1.0493 |
108.9 |
103.8 |
6.47 |
|
12.6 |
1.35187 |
1.0497 |
110.0 |
104.8 |
6.54 |
|
12.7 |
1.35203 |
1.0501 |
111.1 |
105.8 |
6.60 |
|
12.8 |
1.35219 |
1.0506 |
112.2 |
106.8 |
6.67 |
|
12.9 |
1.35234 |
1.0510 |
113.2 |
107.8 |
6.73 |
|
13.0 |
1.35250 |
1.0514 |
114.3 |
108.7 |
6.79 |
|
13.1 |
1.35266 |
1.0518 |
115.4 |
109.7 |
6.86 |
|
13.2 |
1.35282 |
1.0522 |
116.5 |
110.7 |
6.92 |
|
13.3 |
1.35298 |
1.0527 |
117.6 |
111.7 |
6.99 |
|
13.4 |
1.35313 |
1.0531 |
118.7 |
112.7 |
7.05 |
|
13.5 |
1.35329 |
1.0535 |
119.7 |
113.7 |
7.11 |
|
13.6 |
1.35345 |
1.0539 |
120.8 |
114.7 |
7.18 |
|
13.7 |
1.35361 |
1.0543 |
121.9 |
115.6 |
7.24 |
|
13.8 |
1.35377 |
1.0548 |
123.0 |
116.6 |
7.31 |
|
13.9 |
1.35393 |
1.0552 |
124.1 |
117.6 |
7.38 |
|
14.0 |
1.35408 |
1.0556 |
125.2 |
118.6 |
7.44 |
|
14.1 |
1.35424 |
1.0560 |
126.3 |
119.6 |
7.51 |
|
14.2 |
1.35440 |
1.0564 |
127.4 |
120.6 |
7.57 |
|
14.3 |
1.35456 |
1.0569 |
128.5 |
121.6 |
7.64 |
|
14.4 |
1.35472 |
1.0573 |
129.6 |
122.5 |
7.70 |
|
14.5 |
1.35488 |
1.0577 |
130.6 |
123.5 |
7.76 |
|
14.6 |
1.35504 |
1.0581 |
131.7 |
124.5 |
7.83 |
|
14.7 |
1.35520 |
1.0586 |
132.8 |
125.5 |
7.89 |
|
14.8 |
1.35536 |
1.0590 |
133.9 |
126.5 |
7.96 |
|
14.9 |
1.35552 |
1.0594 |
135.0 |
127.5 |
8.02 |
|
15.0 |
1.35568 |
1.0598 |
136.1 |
128.4 |
8.09 |
|
15.1 |
1.35584 |
1.0603 |
137.2 |
129.4 |
8.15 |
|
15.2 |
1.35600 |
1.0607 |
138.3 |
130.4 |
8.22 |
|
15.3 |
1.35616 |
1.0611 |
139.4 |
131.4 |
8.28 |
|
15.4 |
1.35632 |
1.0616 |
140.5 |
132.4 |
8.35 |
|
15.5 |
1.35648 |
1.0620 |
141.6 |
133.4 |
8.42 |
|
15.6 |
1.35664 |
1.0624 |
142.7 |
134.3 |
8.48 |
|
15.7 |
1.35680 |
1.0628 |
143.8 |
135.3 |
8.55 |
|
15.8 |
1.35696 |
1.0633 |
144.9 |
136.3 |
8.61 |
|
15.9 |
1.35713 |
1.0637 |
146.0 |
137.3 |
8.68 |
|
16.0 |
1.35729 |
1.0641 |
147.1 |
138.3 |
8.74 |
|
16.1 |
1.35745 |
1.0646 |
148.2 |
139.3 |
8.81 |
|
16.2 |
1.35761 |
1.0650 |
149.3 |
140.2 |
8.87 |
|
16.3 |
1.35777 |
1.0654 |
150.5 |
141.2 |
8.94 |
|
16.4 |
1.35793 |
1.0659 |
151.6 |
142.2 |
9.01 |
|
16.5 |
1.35810 |
1.0663 |
152.7 |
143.2 |
9.07 |
|
16.6 |
1.35826 |
1.0667 |
153.8 |
144.2 |
9.14 |
|
16.7 |
1.35842 |
1.0672 |
154.9 |
145.1 |
9.21 |
|
16.8 |
1.35858 |
1.0676 |
156.0 |
146.1 |
9.27 |
|
16.9 |
1.35874 |
1.0680 |
157.1 |
147.1 |
9.34 |
|
17.0 |
1.35891 |
1.0685 |
158.2 |
148.1 |
9.40 |
|
17.1 |
1.35907 |
1.0689 |
159.3 |
149.1 |
9.47 |
|
17.2 |
1.35923 |
1.0693 |
160.4 |
150.0 |
9.53 |
|
17.3 |
1.35940 |
1.0698 |
161.6 |
151.0 |
9.60 |
|
17.4 |
1.35956 |
1.0702 |
162.7 |
152.0 |
9.67 |
|
17.5 |
1.35972 |
1.0707 |
163.8 |
153.0 |
9.73 |
|
17.6 |
1.35989 |
1.0711 |
164.9 |
154.0 |
9.80 |
|
17.7 |
1.36005 |
1.0715 |
166.0 |
154.9 |
9.87 |
|
17.8 |
1.36021 |
1.0720 |
167.1 |
155.9 |
9.93 |
|
17.9 |
1.36038 |
1.0724 |
168.3 |
156.9 |
10.00 |
|
18.0 |
1.36054 |
1.0729 |
169.4 |
157.9 |
10.07 |
|
18.1 |
1.36070 |
1.0733 |
170.5 |
158.9 |
10.13 |
|
18.2 |
1.36087 |
1.0737 |
171.6 |
159.8 |
10.20 |
|
18.3 |
1.36103 |
1.0742 |
172.7 |
160.8 |
10.26 |
|
18.4 |
1.36120 |
1.0746 |
173.9 |
161.8 |
10.33 |
|
18.5 |
1.36136 |
1.0751 |
175.0 |
162.8 |
10.40 |
|
18.6 |
1.36153 |
1.0755 |
176.1 |
163.7 |
10.47 |
|
18.7 |
1.36169 |
1.0760 |
177.2 |
164.7 |
10.53 |
|
18.8 |
1.36185 |
1.0764 |
178.4 |
165.7 |
10.60 |
|
18.9 |
1.36202 |
1.0768 |
179.5 |
166.7 |
10.67 |
|
19.0 |
1.36219 |
1.0773 |
180.6 |
167.6 |
10.73 |
|
19.1 |
1.36235 |
1.0777 |
181.7 |
168.6 |
10.80 |
|
19.2 |
1.36252 |
1.0782 |
182.9 |
169.6 |
10.87 |
|
19.3 |
1.36268 |
1.0786 |
184.0 |
170.6 |
10.94 |
|
19.4 |
1.36285 |
1.0791 |
185.1 |
171.5 |
11.00 |
|
19.5 |
1.36301 |
1.0795 |
186.2 |
172.5 |
11.07 |
|
19.6 |
1.36318 |
1.0800 |
187.4 |
173.5 |
11.14 |
|
19.7 |
1.36334 |
1.0804 |
188.5 |
174.5 |
11.20 |
|
19.8 |
1.36351 |
1.0809 |
189.6 |
175.4 |
11.27 |
|
19.9 |
1.36368 |
1.0813 |
190.8 |
176.4 |
11.34 |
|
20.0 |
1.36384 |
1.0818 |
191.9 |
177.4 |
11.40 |
|
20.1 |
1.36401 |
1.0822 |
193.0 |
178.4 |
11.47 |
|
20.2 |
1.36418 |
1.0827 |
194.2 |
179.3 |
11.54 |
|
20.3 |
1.36434 |
1.0831 |
195.3 |
180.3 |
11.61 |
|
20.4 |
1.36451 |
1.0836 |
196.4 |
181.3 |
11.67 |
|
20.5 |
1.36468 |
1.0840 |
197.6 |
182.3 |
11.74 |
|
20.6 |
1.36484 |
1.0845 |
198.7 |
183.2 |
11.81 |
|
20.7 |
1.36501 |
1.0849 |
199.8 |
184.2 |
11.87 |
|
20.8 |
1.36518 |
1.0854 |
201.0 |
185.2 |
11.95 |
|
20.9 |
1.36535 |
1.0858 |
202.1 |
186.1 |
12.01 |
|
21.0 |
1.36551 |
1.0863 |
203.3 |
187.1 |
12.08 |
|
21.1 |
1.36568 |
1.0867 |
204.4 |
188.1 |
12.15 |
|
21.2 |
1.36585 |
1.0872 |
205.5 |
189.1 |
12.21 |
|
21.3 |
1.36602 |
1.0876 |
206.7 |
190.0 |
12.28 |
|
21.4 |
1.36619 |
1.0881 |
207.8 |
191.0 |
12.35 |
|
21.5 |
1.36635 |
1.0885 |
209.0 |
192.0 |
12.42 |
|
21.6 |
1.36652 |
1.0890 |
210.1 |
192.9 |
12.49 |
|
21.7 |
1.36669 |
1.0895 |
211.3 |
193.9 |
12.56 |
|
21.8 |
1.36686 |
1.0899 |
212.4 |
194.9 |
12.62 |
|
21.9 |
1.36703 |
1.0904 |
213.6 |
195.9 |
12.69 |
|
22.0 |
1.36720 |
1.0908 |
214.7 |
196.8 |
12.76 |
|
22.1 |
1.36737 |
1.0913 |
215.9 |
197.8 |
12.83 |
|
22.2 |
1.36754 |
1.0917 |
217.0 |
198.8 |
12.90 |
|
22.3 |
1.36771 |
1.0922 |
218.2 |
199.7 |
12.97 |
|
22.4 |
1.36787 |
1.0927 |
219.3 |
200.7 |
13.03 |
|
22.5 |
1.36804 |
1.0931 |
220.5 |
201.7 |
13.10 |
|
22.6 |
1.36821 |
1.0936 |
221.6 |
202.6 |
13.17 |
|
22.7 |
1.36838 |
1.0940 |
222.8 |
203.6 |
13.24 |
|
22.8 |
1.36855 |
1.0945 |
223.9 |
204.6 |
13.31 |
|
22.9 |
1.36872 |
1.0950 |
225.1 |
205.5 |
13.38 |
|
23.0 |
1.36889 |
1.0954 |
226.2 |
206.5 |
13.44 |
|
23.1 |
1.36906 |
1.0959 |
227.4 |
207.5 |
13.51 |
|
23.2 |
1.36924 |
1.0964 |
228.5 |
208.4 |
13.58 |
|
23.3 |
1.36941 |
1.0968 |
229.7 |
209.4 |
13.65 |
|
23.4 |
1.36958 |
1.0973 |
230.8 |
210.4 |
13.72 |
|
23.5 |
1.36975 |
1.0977 |
232.0 |
211.3 |
13.79 |
|
23.6 |
1.36992 |
1.0982 |
233.2 |
212.3 |
13.86 |
|
23.7 |
1.37009 |
1.0987 |
234.3 |
213.3 |
13.92 |
|
23.8 |
1.37026 |
1.0991 |
235.5 |
214.2 |
14.00 |
|
23.9 |
1.37043 |
1.0996 |
236.6 |
215.2 |
14.06 |
|
24.0 |
1.37060 |
1.1001 |
237.8 |
216.2 |
14.13 |
|
24.1 |
1.37078 |
1.1005 |
239.0 |
217.1 |
14.20 |
|
24.2 |
1.37095 |
1.1010 |
240.1 |
218.1 |
14.27 |
|
24.3 |
1.37112 |
1.1015 |
241.3 |
219.1 |
14.34 |
|
24.4 |
1.37129 |
1.1019 |
242.5 |
220.0 |
14.41 |
|
24.5 |
1.37146 |
1.1024 |
243.6 |
221.0 |
14.48 |
|
24.6 |
1.37164 |
1.1029 |
244.8 |
222.0 |
14.55 |
|
24.7 |
1.37181 |
1.1033 |
246.0 |
222.9 |
14.62 |
|
24.8 |
1.37198 |
1.1038 |
247.1 |
223.9 |
14.69 |
|
24.9 |
1.37216 |
1.1043 |
248.3 |
224.8 |
14.76 |
|
25.0 |
1.37233 |
1.1047 |
249.5 |
225.8 |
14.83 |
|
25.1 |
1.37250 |
1.1052 |
250.6 |
226.8 |
14.89 |
|
25.2 |
1.37267 |
1.1057 |
251.8 |
227.7 |
14.96 |
|
25.3 |
1.37285 |
1.1062 |
253.0 |
228.7 |
15.04 |
|
25.4 |
1.37302 |
1.1066 |
254.1 |
229.7 |
15.10 |
|
25.5 |
1.37319 |
1.1071 |
255.3 |
230.6 |
15.17 |
|
25.6 |
1.37337 |
1.1076 |
256.5 |
231.6 |
15.24 |
|
25.7 |
1.37354 |
1.1080 |
257.7 |
232.5 |
15.32 |
|
25.8 |
1.37372 |
1.1085 |
258.8 |
233.5 |
15.38 |
|
25.9 |
1.37389 |
1.1090 |
260.0 |
234.5 |
15.45 |
|
26.0 |
1.37407 |
1.1095 |
261.2 |
235.4 |
15.52 |
|
26.1 |
1.37424 |
1.1099 |
262.4 |
236.4 |
15.59 |
|
26.2 |
1.37441 |
1.1104 |
263.6 |
237.3 |
15.67 |
|
26.3 |
1.37459 |
1.1109 |
264.7 |
238.3 |
15.73 |
|
26.4 |
1.37476 |
1.1114 |
265.9 |
239.3 |
15.80 |
|
26.5 |
1.37494 |
1.1118 |
267.1 |
240.2 |
15.87 |
|
26.6 |
1.37511 |
1.1123 |
268.3 |
241.2 |
15.95 |
|
26.7 |
1.37529 |
1.1128 |
269.5 |
242.1 |
16.02 |
|
26.8 |
1.37546 |
1.1133 |
270.6 |
243.1 |
16.08 |
|
26.9 |
1.37564 |
1.1138 |
271.8 |
244.1 |
16.15 |
|
27.0 |
1.37582 |
1.1142 |
273.0 |
245.0 |
16.22 |
|
27.1 |
1.37599 |
1.1147 |
274.2 |
246.0 |
16.30 |
|
27.2 |
1.37617 |
1.1152 |
275.4 |
246.9 |
16.37 |
|
27.3 |
1.37634 |
1.1157 |
276.6 |
247.9 |
16.44 |
|
27.4 |
1.37652 |
1.1161 |
277.8 |
248.9 |
16.51 |
|
27.5 |
1.37670 |
1.1166 |
278.9 |
249.8 |
16.58 |
|
27.6 |
1.37687 |
1.1171 |
280.1 |
250.8 |
16.65 |
|
27.7 |
1.37705 |
1.1176 |
281.3 |
251.7 |
16.72 |
|
27.8 |
1.37723 |
1.1181 |
282.5 |
252.7 |
16.79 |
|
27.9 |
1.37740 |
1.1185 |
283.7 |
253.6 |
16.86 |
|
28.0 |
1.37758 |
1.1190 |
284.9 |
254.6 |
16.93 |
|
28.1 |
1.37776 |
1.1195 |
286.1 |
255.5 |
17.00 |
|
28.2 |
1.37793 |
1.1200 |
287.3 |
256.5 |
17.07 |
|
28.3 |
1.37811 |
1.1205 |
288.5 |
257.5 |
17.15 |
|
28.4 |
1.37829 |
1.1210 |
289.7 |
258.4 |
17.22 |
|
28.5 |
1.37847 |
1.1214 |
290.9 |
259.4 |
17.29 |
|
28.6 |
1.37864 |
1.1219 |
292.1 |
260.3 |
17.36 |
|
28.7 |
1.37882 |
1.1224 |
293.3 |
261.3 |
17.43 |
|
28.8 |
1.37900 |
1.1229 |
294.5 |
262.2 |
17.50 |
|
28.9 |
1.37918 |
1.1234 |
295.7 |
263.2 |
17.57 |
|
29.0 |
1.37936 |
1.1239 |
296.9 |
264.2 |
17.64 |
|
29.1 |
1.37954 |
1.1244 |
298.1 |
265.1 |
17.72 |
|
29.2 |
1.37972 |
1.1248 |
299.3 |
266.1 |
17.79 |
|
29.3 |
1.37989 |
1.1253 |
300.5 |
267.0 |
17.86 |
|
29.4 |
1.38007 |
1.1258 |
301.7 |
268.0 |
17.93 |
|
29.5 |
1.38025 |
1.1263 |
302.9 |
268.9 |
18.00 |
|
29.6 |
1.38043 |
1.1268 |
304.1 |
269.9 |
18.07 |
|
29.7 |
1.38061 |
1.1273 |
305.3 |
270.8 |
18.14 |
|
29.8 |
1.38079 |
1.1278 |
306.5 |
271.8 |
18.22 |
|
29.9 |
1.38097 |
1.1283 |
307.7 |
272.7 |
18.29 |
|
30.0 |
1.38115 |
1.1287 |
308.9 |
273.7 |
18.36 |
|
30.1 |
1.38133 |
1.1292 |
310.1 |
274.6 |
18.43 |
|
30.2 |
1.38151 |
1.1297 |
311.3 |
275.6 |
18.50 |
|
30.3 |
1.38169 |
1.1302 |
312.6 |
276.5 |
18.58 |
|
30.4 |
1.38187 |
1.1307 |
313.8 |
277.5 |
18.65 |
|
30.5 |
1.38205 |
1.1312 |
315.0 |
278.5 |
18.72 |
|
30.6 |
1.38223 |
1.1317 |
316.2 |
279.4 |
18.79 |
|
30.7 |
1.38241 |
1.1322 |
317.4 |
280.4 |
18.86 |
|
30.8 |
1.38259 |
1.1327 |
318.6 |
281.3 |
18.93 |
|
30.9 |
1.38277 |
1.1332 |
319.8 |
282.3 |
19.01 |
|
31.0 |
1.38296 |
1.1337 |
321.1 |
283.2 |
19.08 |
|
31.1 |
1.38314 |
1.1342 |
322.3 |
284.2 |
19.15 |
|
31.2 |
1.38332 |
1.1346 |
323.5 |
285.1 |
19.23 |
|
31.3 |
1.38350 |
1.1351 |
324.7 |
286.1 |
19.30 |
|
31.4 |
1.38368 |
1.1356 |
325.9 |
287.0 |
19.37 |
|
31.5 |
1.38386 |
1.1361 |
327.2 |
288.0 |
19.45 |
|
31.6 |
1.38405 |
1.1366 |
328.4 |
288.9 |
19.52 |
|
31.7 |
1.38423 |
1.1371 |
329.6 |
289.9 |
19.59 |
|
31.8 |
1.38441 |
1.1376 |
330.8 |
290.8 |
19.66 |
|
31.9 |
1.38459 |
1.1381 |
332.1 |
291.8 |
19.74 |
|
32.0 |
1.38478 |
1.1386 |
333.3 |
292.7 |
19.81 |
|
32.1 |
1.38496 |
1.1391 |
334.5 |
293.7 |
19.88 |
|
32.2 |
1.38514 |
1.1396 |
335.7 |
294.6 |
19.95 |
|
32.3 |
1.38532 |
1.1401 |
337.0 |
295.6 |
20.03 |
|
32.4 |
1.38551 |
1.1406 |
338.2 |
296.5 |
20.10 |
|
32.5 |
1.38569 |
1.1411 |
339.4 |
297.5 |
20.17 |
|
32.6 |
1.38587 |
1.1416 |
340.7 |
298.4 |
20.25 |
|
32.7 |
1.38606 |
1.1421 |
341.9 |
299.4 |
20.32 |
|
32.8 |
1.38624 |
1.1426 |
343.1 |
300.3 |
20.39 |
|
32.9 |
1.38643 |
1.1431 |
344.4 |
301.3 |
20.47 |
|
33.0 |
1.38661 |
1.1436 |
345.6 |
302.2 |
20.54 |
|
33.1 |
1.38679 |
1.1441 |
346.8 |
303.2 |
20.61 |
|
33.2 |
1.38698 |
1.1446 |
348.1 |
304.1 |
20.69 |
|
33.3 |
1.38716 |
1.1451 |
349.3 |
305.0 |
20.76 |
|
33.4 |
1.38735 |
1.1456 |
350.6 |
306.0 |
20.84 |
|
33.5 |
1.38753 |
1.1461 |
351.8 |
306.9 |
20.91 |
|
33.6 |
1.38772 |
1.1466 |
353.0 |
307.9 |
20.98 |
|
33.7 |
1.38790 |
1.1471 |
354.3 |
308.8 |
21.06 |
|
33.8 |
1.38809 |
1.1476 |
355.5 |
309.8 |
21.13 |
|
33.9 |
1.38827 |
1.1481 |
356.8 |
310.7 |
21.20 |
|
34.0 |
1.38846 |
1.1486 |
358.0 |
311.7 |
21.28 |
|
34.1 |
1.38864 |
1.1491 |
359.2 |
312.6 |
21.35 |
|
34.2 |
1.38883 |
1.1496 |
360.5 |
313.6 |
21.42 |
|
34.3 |
1.38902 |
1.1501 |
361.7 |
314.5 |
21.50 |
|
34.4 |
1.38920 |
1.1507 |
363.0 |
315.5 |
21.57 |
|
34.5 |
1.38939 |
1.1512 |
364.2 |
316.4 |
21.64 |
|
34.6 |
1.38958 |
1.1517 |
365.5 |
317.4 |
21.72 |
|
34.7 |
1.38976 |
1.1522 |
366.7 |
318.3 |
21.79 |
|
34.8 |
1.38995 |
1.1527 |
368.0 |
319.2 |
21.87 |
|
34.9 |
1.39014 |
1.1532 |
369.2 |
320.2 |
21.94 |
|
35.0 |
1.39032 |
1.1537 |
370.5 |
321.1 |
22.02 |
|
35.1 |
1.39051 |
1.1542 |
371.8 |
322.1 |
22.10 |
|
35.2 |
1.39070 |
1.1547 |
373.0 |
323.0 |
22.17 |
|
35.3 |
1.39088 |
1.1552 |
374.3 |
324.0 |
22.24 |
|
35.4 |
1.39107 |
1.1557 |
375.5 |
324.9 |
22.32 |
|
35.5 |
1.39126 |
1.1563 |
376.8 |
325.9 |
22.39 |
|
35.6 |
1.39145 |
1.1568 |
378.0 |
326.8 |
22.46 |
|
35.7 |
1.39164 |
1.1573 |
379.3 |
327.8 |
22.54 |
|
35.8 |
1.39182 |
1.1578 |
380.6 |
328.7 |
22.62 |
|
35.9 |
1.39201 |
1.1583 |
381.8 |
329.6 |
22.69 |
|
36.0 |
1.39220 |
1.1588 |
383.1 |
330.6 |
22.77 |
|
36.1 |
1.39239 |
1.1593 |
384.4 |
331.5 |
22.84 |
|
36.2 |
1.39258 |
1.1598 |
385.6 |
332.5 |
22.92 |
|
36.3 |
1.39277 |
1.1603 |
386.9 |
333.4 |
22.99 |
|
36.4 |
1.39296 |
1.1609 |
388.1 |
334.4 |
23.06 |
|
36.5 |
1.39314 |
1.1614 |
389.4 |
335.3 |
23.14 |
|
36.6 |
1.39333 |
1.1619 |
390.7 |
336.3 |
23.22 |
|
36.7 |
1.39352 |
1.1624 |
392.0 |
337.2 |
23.30 |
|
36.8 |
1.39371 |
1.1629 |
393.2 |
338.1 |
23.37 |
|
36.9 |
1.39390 |
1.1634 |
394.5 |
339.1 |
23.45 |
|
37.0 |
1.39409 |
1.1640 |
395.8 |
340.0 |
23.52 |
|
37.1 |
1.39428 |
1.1645 |
397.0 |
341.0 |
23.59 |
|
37.2 |
1.39447 |
1.1650 |
398.3 |
341.9 |
23.67 |
|
37.3 |
1.39466 |
1.1655 |
399.6 |
342.9 |
23.75 |
|
37.4 |
1.39485 |
1.1660 |
400.9 |
343.8 |
23.83 |
|
37.5 |
1.39504 |
1.1665 |
402.1 |
344.7 |
23.90 |
|
37.6 |
1.39524 |
1.1671 |
403.4 |
345.7 |
23.97 |
|
37.7 |
1.39543 |
1.1676 |
404.7 |
346.6 |
24.05 |
|
37.8 |
1.39562 |
1.1681 |
406.0 |
347.6 |
24.13 |
|
37.9 |
1.39581 |
1.1686 |
407.3 |
348.5 |
24.21 |
|
38.0 |
1.39600 |
1.1691 |
408.6 |
349.4 |
24.28 |
|
38.1 |
1.39619 |
1.1697 |
409.8 |
350.4 |
24.35 |
|
38.2 |
1.39638 |
1.1702 |
411.1 |
351.3 |
24.43 |
|
38.3 |
1.39658 |
1.1707 |
412.4 |
352.3 |
24.51 |
|
38.4 |
1.39677 |
1.1712 |
413.7 |
353.2 |
24.59 |
|
38.5 |
1.39696 |
1.1717 |
415.0 |
354.2 |
24.66 |
|
38.6 |
1.39715 |
1.1723 |
416.3 |
355.1 |
24.74 |
|
38.7 |
1.39734 |
1.1728 |
417.6 |
356.0 |
24.82 |
|
38.8 |
1.39754 |
1.1733 |
418.8 |
357.0 |
24.89 |
|
38.9 |
1.39773 |
1.1738 |
420.1 |
357.9 |
24.97 |
|
39.0 |
1.39792 |
1.1744 |
421.4 |
358.9 |
25.04 |
|
39.1 |
1.39812 |
1.1749 |
422.7 |
359.8 |
25.12 |
|
39.2 |
1.39831 |
1.1754 |
424.0 |
360.7 |
25.20 |
|
39.3 |
1.39850 |
1.1759 |
425.3 |
361.7 |
25.28 |
|
39.4 |
1.39870 |
1.1765 |
426.6 |
362.6 |
25.35 |
|
39.5 |
1.39889 |
1.1770 |
427.9 |
363.6 |
25.43 |
|
39.6 |
1.39908 |
1.1775 |
429.2 |
364.5 |
25.51 |
|
39.7 |
1.39928 |
1.1780 |
430.5 |
365.4 |
25.58 |
|
39.8 |
1.39947 |
1.1786 |
431.8 |
366.4 |
25.66 |
|
39.9 |
1.39967 |
1.1791 |
433.1 |
367.3 |
25.74 |
|
40.0 |
1.39986 |
1.1796 |
434.4 |
368.3 |
25.82 |
|
40.1 |
1.40006 |
1.1801 |
435.7 |
369.2 |
25.89 |
|
40.2 |
1.40025 |
1.1807 |
437.0 |
370.1 |
25.97 |
|
40.3 |
1.40044 |
1.1812 |
438.3 |
371.1 |
26.05 |
|
40.4 |
1.40064 |
1.1817 |
439.6 |
372.0 |
26.13 |
|
40.5 |
1.40083 |
1.1823 |
440.9 |
373.0 |
26.20 |
|
40.6 |
1.40103 |
1.1828 |
442.2 |
373.9 |
26.28 |
|
40.7 |
1.40123 |
1.1833 |
443.6 |
374.8 |
26.36 |
|
40.8 |
1.40142 |
1.1839 |
444.9 |
375.8 |
26.44 |
|
40.9 |
1.40162 |
1.1844 |
446.2 |
376.7 |
26.52 |
|
41.0 |
1.40181 |
1.1849 |
447.5 |
377.7 |
26.59 |
|
41.1 |
1.40201 |
1.1855 |
448.8 |
378.6 |
26.67 |
|
41.2 |
1.40221 |
1.1860 |
450.1 |
379.5 |
26.75 |
|
41.3 |
1.40240 |
1.1865 |
451.4 |
380.5 |
26.83 |
|
41.4 |
1.40260 |
1.1871 |
452.8 |
381.4 |
26.91 |
|
41.5 |
1.40280 |
1.1876 |
454.1 |
382.3 |
26.99 |
|
41.6 |
1.40299 |
1.1881 |
455.4 |
383.3 |
27.06 |
|
41.7 |
1.40319 |
1.1887 |
456.7 |
384.2 |
27.14 |
|
41.8 |
1.40339 |
1.1892 |
458.0 |
385.2 |
27.22 |
|
41.9 |
1.40358 |
1.1897 |
459.4 |
386.1 |
27.30 |
|
42.0 |
1.40378 |
1.1903 |
460.7 |
387.0 |
27.38 |
|
42.1 |
1.40398 |
1.1908 |
462.0 |
388.0 |
27.46 |
|
42.2 |
1.40418 |
1.1913 |
463.3 |
388.9 |
27.53 |
|
42.3 |
1.40437 |
1.1919 |
464.7 |
389.9 |
27.62 |
|
42.4 |
1.40457 |
1.1924 |
466.0 |
390.8 |
27.69 |
|
42.5 |
1.40477 |
1.1929 |
467.3 |
391.7 |
27.77 |
|
42.6 |
1.40497 |
1.1935 |
468.6 |
392.7 |
27.85 |
|
42.7 |
1.40517 |
1.1940 |
470.0 |
393.6 |
27.93 |
|
42.8 |
1.40537 |
1.1946 |
471.3 |
394.5 |
28.01 |
|
42.9 |
1.40557 |
1.1951 |
472.6 |
395.5 |
28.09 |
|
43.0 |
1.40576 |
1.1956 |
474.0 |
396.4 |
28.17 |
|
43.1 |
1.40596 |
1.1962 |
475.3 |
397.3 |
28.25 |
|
43.2 |
1.40616 |
1.1967 |
476.6 |
398.3 |
28.32 |
|
43.3 |
1.40636 |
1.1973 |
478.0 |
399.2 |
28.41 |
|
43.4 |
1.40656 |
1.1978 |
479.3 |
400.2 |
28.48 |
|
43.5 |
1.40676 |
1.1983 |
480.7 |
401.1 |
28.57 |
|
43.6 |
1.40696 |
1.1989 |
482.0 |
402.0 |
28.65 |
|
43.7 |
1.40716 |
1.1994 |
483.3 |
403.0 |
28.72 |
|
43.8 |
1.40736 |
1.2000 |
484.7 |
403.9 |
28.81 |
|
43.9 |
1.40756 |
1.2005 |
486.0 |
404.8 |
28.88 |
|
44.0 |
1.40776 |
1.2011 |
487.4 |
405.8 |
28.97 |
|
44.1 |
1.40796 |
1.2016 |
488.7 |
406.7 |
29.04 |
|
44.2 |
1.40817 |
1.2022 |
490.1 |
407.6 |
29.13 |
|
44.3 |
1.40837 |
1.2027 |
491.4 |
408.6 |
29.20 |
|
44.4 |
1.40857 |
1.2032 |
492.8 |
409.5 |
29.29 |
|
44.5 |
1.40877 |
1.2038 |
494.1 |
410.4 |
29.36 |
|
44.6 |
1.40897 |
1.2043 |
495.5 |
411.4 |
29.45 |
|
44.7 |
1.40917 |
1.2049 |
496.8 |
412.3 |
29.52 |
|
44.8 |
1.40937 |
1.2054 |
498.2 |
413.3 |
29.61 |
|
44.9 |
1.40958 |
1.2060 |
499.5 |
414.2 |
29.69 |
|
45.0 |
1.40978 |
1.2065 |
500.9 |
415.1 |
29.77 |
|
45.1 |
1.40998 |
1.2071 |
502.2 |
416.1 |
29.85 |
|
45.2 |
1.41018 |
1.2076 |
503.6 |
417.0 |
29.93 |
|
45.3 |
1.41039 |
1.2082 |
504.9 |
417.9 |
30.01 |
|
45.4 |
1.41059 |
1.2087 |
506.3 |
418.9 |
30.09 |
|
45.5 |
1.41079 |
1.2093 |
507.7 |
419.8 |
30.17 |
|
45.6 |
1.41099 |
1.2098 |
509.0 |
420.7 |
30.25 |
|
45.7 |
1.41120 |
1.2104 |
510.4 |
421.7 |
30.33 |
|
45.8 |
1.41140 |
1.2109 |
511.7 |
422.6 |
30.41 |
|
45.9 |
1.41160 |
1.2115 |
513.1 |
423.5 |
30.49 |
|
46.0 |
1.41181 |
1.2120 |
514.5 |
424.5 |
30.58 |
|
46.1 |
1.41201 |
1.2126 |
515.8 |
425.4 |
30.65 |
|
46.2 |
1.41222 |
1.2131 |
517.2 |
426.3 |
30.74 |
|
46.3 |
1.41242 |
1.2137 |
518.6 |
427.3 |
30.82 |
|
46.4 |
1.41262 |
1.2142 |
519.9 |
428.2 |
30.90 |
|
46.5 |
1.41283 |
1.2148 |
521.3 |
429.1 |
30.98 |
|
46.6 |
1.41303 |
1.2154 |
522.7 |
430.1 |
31.06 |
|
46.7 |
1.41324 |
1.2159 |
524.1 |
431.0 |
31.15 |
|
46.8 |
1.41344 |
1.2165 |
525.4 |
431.9 |
31.22 |
|
46.9 |
1.41365 |
1.2170 |
526.8 |
432.9 |
31.31 |
|
47.0 |
1.41385 |
1.2176 |
528.2 |
433.8 |
31.39 |
|
47.1 |
1.41406 |
1.2181 |
529.6 |
434.7 |
31.47 |
|
47.2 |
1.41427 |
1.2187 |
530.9 |
435.7 |
31.55 |
|
47.3 |
1.41447 |
1.2192 |
532.3 |
436.6 |
31.63 |
|
47.4 |
1.41468 |
1.2198 |
533.7 |
437.5 |
31.72 |
|
47.5 |
1.41488 |
1.2204 |
535.1 |
438.5 |
31.80 |
|
47.6 |
1.41509 |
1.2209 |
536.5 |
439.4 |
31.88 |
|
47.7 |
1.41530 |
1.2215 |
537.9 |
440.3 |
31.97 |
|
47.8 |
1.41550 |
1.2220 |
539.2 |
441.3 |
32.04 |
|
47.9 |
1.41571 |
1.2226 |
540.6 |
442.2 |
32.13 |
|
48.0 |
1.41592 |
1.2232 |
542.0 |
443.1 |
32.21 |
|
48.1 |
1.41612 |
1.2237 |
543.4 |
444.1 |
32.29 |
|
48.2 |
1.41633 |
1.2243 |
544.8 |
445.0 |
32.38 |
|
48.3 |
1.41654 |
1.2248 |
546.2 |
445.9 |
32.46 |
|
48.4 |
1.41674 |
1.2254 |
547.6 |
446.8 |
32.54 |
|
48.5 |
1.41695 |
1.2260 |
549.0 |
447.8 |
32.63 |
|
48.6 |
1.41716 |
1.2265 |
550.4 |
448.7 |
32.71 |
|
48.7 |
1.41737 |
1.2271 |
551.8 |
449.6 |
32.79 |
|
48.8 |
1.41758 |
1.2277 |
553.2 |
450.6 |
32.88 |
|
48.9 |
1.41779 |
1.2282 |
554.6 |
451.5 |
32.96 |
|
49.0 |
1.41799 |
1.2288 |
556.0 |
452.4 |
33.04 |
|
49.1 |
1.41820 |
1.2294 |
557.4 |
453.4 |
33.13 |
|
49.2 |
1.41841 |
1.2299 |
558.8 |
454.3 |
33.21 |
|
49.3 |
1.41862 |
1.2305 |
560.2 |
455.2 |
33.29 |
|
49.4 |
1.41883 |
1.2311 |
561.6 |
456.2 |
33.38 |
|
49.5 |
1.41904 |
1.2316 |
563.0 |
457.1 |
33.46 |
|
49.6 |
1.41925 |
1.2322 |
564.4 |
458.0 |
33.54 |
|
49.7 |
1.41946 |
1.2328 |
565.8 |
458.9 |
33.63 |
|
49.8 |
1.41967 |
1.2333 |
567.2 |
459.9 |
33.71 |
|
49.9 |
1.41988 |
1.2339 |
568.6 |
460.8 |
33.79 |
|
50.0 |
1.42009 |
1.2345 |
570.0 |
461.7 |
33.88 |
|
50.1 |
1.42030 |
1.2350 |
571.4 |
462.7 |
33.96 |
|
50.2 |
1.42051 |
1.2356 |
572.8 |
463.6 |
34.04 |
|
50.3 |
1.42072 |
1.2362 |
574.2 |
464.5 |
34.12 |
|
50.4 |
1.42093 |
1.2368 |
575.6 |
465.4 |
34.21 |
|
50.5 |
1.42114 |
1.2373 |
577.1 |
466.4 |
34.30 |
|
50.6 |
1.42135 |
1.2379 |
578.5 |
467.3 |
34.38 |
|
50.7 |
1.42156 |
1.2385 |
579.9 |
468.2 |
34.46 |
|
50.8 |
1.42177 |
1.2390 |
581.3 |
469.2 |
34.55 |
|
50.9 |
1.42199 |
1.2396 |
582.7 |
470.1 |
34.63 |
|
51.0 |
1.42220 |
1.2402 |
584.2 |
471.0 |
34.72 |
|
51.1 |
1.42241 |
1.2408 |
585.6 |
471.9 |
34.80 |
|
51.2 |
1.42262 |
1.2413 |
587.0 |
472.9 |
34.89 |
|
51.3 |
1.42283 |
1.2419 |
588.4 |
473.8 |
34.97 |
|
51.4 |
1.42305 |
1.2425 |
589.9 |
474.7 |
35.06 |
|
51.5 |
1.42326 |
1.2431 |
591.3 |
475.7 |
35.14 |
|
51.6 |
1.42347 |
1.2436 |
592.7 |
476.6 |
35.22 |
|
51.7 |
1.42368 |
1.2442 |
594.1 |
477.5 |
35.31 |
|
51.8 |
1.42390 |
1.2448 |
595.6 |
478.4 |
35.40 |
|
51.9 |
1.42411 |
1.2454 |
597.0 |
479.4 |
35.48 |
|
52.0 |
1.42432 |
1.2460 |
598.4 |
480.3 |
35.56 |
|
52.1 |
1.42454 |
1.2465 |
599.9 |
481.2 |
35.65 |
|
52.2 |
1.42475 |
1.2471 |
601.3 |
482.1 |
35.74 |
|
52.3 |
1.42496 |
1.2477 |
602.7 |
483.1 |
35.82 |
|
52.4 |
1.42518 |
1.2483 |
604.2 |
484.0 |
35.91 |
|
52.5 |
1.42539 |
1.2488 |
605.6 |
484.9 |
35.99 |
|
52.6 |
1.42561 |
1.2494 |
607.0 |
485.8 |
36.07 |
|
52.7 |
1.42582 |
1.2500 |
608.5 |
486.8 |
36.16 |
|
52.8 |
1.42604 |
1.2506 |
609.9 |
487.7 |
36.25 |
|
52.9 |
1.42625 |
1.2512 |
611.4 |
488.6 |
36.34 |
|
53.0 |
1.42647 |
1.2518 |
612.8 |
489.5 |
36.42 |
|
53.1 |
1.42668 |
1.2523 |
614.2 |
490.5 |
36.50 |
|
53.2 |
1.42690 |
1.2529 |
615.7 |
491.4 |
36.59 |
|
53.3 |
1.42711 |
1.2535 |
617.1 |
492.3 |
36.67 |
|
53.4 |
1.42733 |
1.2541 |
618.6 |
493.2 |
36.76 |
|
53.5 |
1.42754 |
1.2547 |
620.0 |
494.2 |
36.85 |
|
53.6 |
1.42776 |
1.2553 |
621.5 |
495.1 |
36.94 |
|
53.7 |
1.42798 |
1.2558 |
622.9 |
496.0 |
37.02 |
|
53.8 |
1.42819 |
1.2564 |
624.4 |
496.9 |
37.11 |
|
53.9 |
1.42841 |
1.2570 |
625.8 |
497.9 |
37.19 |
|
54.0 |
1.42863 |
1.2576 |
627.3 |
498.8 |
37.28 |
|
54.1 |
1.42884 |
1.2582 |
628.7 |
499.7 |
37.36 |
|
54.2 |
1.42906 |
1.2588 |
630.2 |
500.6 |
37.45 |
|
54.3 |
1.42928 |
1.2594 |
631.7 |
501.6 |
37.54 |
|
54.4 |
1.42949 |
1.2600 |
633.1 |
502.5 |
37.63 |
|
54.5 |
1.42971 |
1.2606 |
634.6 |
503.4 |
37.71 |
|
54.6 |
1.42993 |
1.2611 |
636.0 |
504.3 |
37.80 |
|
54.7 |
1.43015 |
1.2617 |
637.5 |
505.2 |
37.89 |
|
54.8 |
1.43036 |
1.2623 |
639.0 |
506.2 |
37.98 |
|
54.9 |
1.43058 |
1.2629 |
640.4 |
507.1 |
38.06 |
|
55.0 |
1.43080 |
1.2635 |
641.9 |
508.0 |
38.15 |
|
55.1 |
1.43102 |
1.2641 |
643.4 |
508.9 |
38.24 |
|
55.2 |
1.43124 |
1.2647 |
644.8 |
509.9 |
38.32 |
|
55.3 |
1.43146 |
1.2653 |
646.3 |
510.8 |
38.41 |
|
55.4 |
1.43168 |
1.2659 |
647.8 |
511.7 |
38.50 |
|
55.5 |
1.43189 |
1.2665 |
649.2 |
512.6 |
38.58 |
|
55.6 |
1.43211 |
1.2671 |
650.7 |
513.5 |
38.67 |
|
55.7 |
1.43233 |
1.2677 |
652.2 |
514.5 |
38.76 |
|
55.8 |
1.43255 |
1.2683 |
653.7 |
515.4 |
38.85 |
|
55.9 |
1.43277 |
1.2689 |
655.1 |
516.3 |
38.93 |
|
56.0 |
1.43299 |
1.2695 |
656.6 |
517.2 |
39.02 |
|
56.1 |
1.43321 |
1.2701 |
658.1 |
518.1 |
39.11 |
|
56.2 |
1.43343 |
1.2706 |
659.6 |
519.1 |
39.20 |
|
56.3 |
1.43365 |
1.2712 |
661.0 |
520.0 |
39.28 |
|
56.4 |
1.43387 |
1.2718 |
662.5 |
520.9 |
39.37 |
|
56.5 |
1.43410 |
1.2724 |
664.0 |
521.8 |
39.46 |
|
56.6 |
1.43432 |
1.2730 |
665.5 |
522.7 |
39.55 |
|
56.7 |
1.43454 |
1.2736 |
667.0 |
523.7 |
39.64 |
|
56.8 |
1.43476 |
1.2742 |
668.5 |
524.6 |
39.73 |
|
56.9 |
1.43498 |
1.2748 |
669.9 |
525.5 |
39.81 |
|
57.0 |
1.43520 |
1.2754 |
671.4 |
526.4 |
39.90 |
|
57.1 |
1.43542 |
1.2760 |
672.9 |
527.3 |
39.99 |
|
57.2 |
1.43565 |
1.2766 |
674.4 |
528.3 |
40.08 |
|
57.3 |
1.43587 |
1.2773 |
675.9 |
529.2 |
40.17 |
|
57.4 |
1.43609 |
1.2779 |
677.4 |
530.1 |
40.26 |
|
57.5 |
1.43631 |
1.2785 |
678.9 |
531.0 |
40.35 |
|
57.6 |
1.43653 |
1.2791 |
680.4 |
531.9 |
40.44 |
|
57.7 |
1.43676 |
1.2797 |
681.9 |
532.8 |
40.53 |
|
57.8 |
1.43698 |
1.2803 |
683.4 |
533.8 |
40.61 |
|
57.9 |
1.43720 |
1.2809 |
684.9 |
534.7 |
40.70 |
|
58.0 |
1.43743 |
1.2815 |
686.4 |
535.6 |
40.79 |
|
58.1 |
1.43765 |
1.2821 |
687.9 |
536.5 |
40.88 |
|
58.2 |
1.43787 |
1.2827 |
689.4 |
537.4 |
40.97 |
|
58.3 |
1.43810 |
1.2833 |
690.9 |
538.3 |
41.06 |
|
58.4 |
1.43832 |
1.2839 |
692.4 |
539.3 |
41.15 |
|
58.5 |
1.43855 |
1.2845 |
693.9 |
540.2 |
41.24 |
|
58.6 |
1.43877 |
1.2851 |
695.4 |
541.1 |
41.33 |
|
58.7 |
1.43899 |
1.2857 |
696.9 |
542.0 |
41.42 |
|
58.8 |
1.43922 |
1.2863 |
698.4 |
542.9 |
41.51 |
|
58.9 |
1.43944 |
1.2870 |
699.9 |
543.8 |
41.60 |
|
59.0 |
1.43967 |
1.2876 |
701.4 |
544.8 |
41.68 |
|
59.1 |
1.43989 |
1.2882 |
702.9 |
545.7 |
41.77 |
|
59.2 |
1.44012 |
1.2888 |
704.4 |
546.6 |
41.86 |
|
59.3 |
1.44035 |
1.2894 |
706.0 |
547.5 |
41.96 |
|
59.4 |
1.44057 |
1.2900 |
707.5 |
548.4 |
42.05 |
|
59.5 |
1.44080 |
1.2906 |
709.0 |
549.3 |
42.14 |
|
59.6 |
1.44102 |
1.2912 |
710.5 |
550.2 |
42.23 |
|
59.7 |
1.44125 |
1.2919 |
712.0 |
551.1 |
42.31 |
|
59.8 |
1.44148 |
1.2925 |
713.5 |
552.1 |
42.40 |
|
59.9 |
1.44170 |
1.2931 |
715.1 |
553.0 |
42.50 |
|
60.0 |
1.44193 |
1.2937 |
716.6 |
553.9 |
42.59 |
|
60.1 |
1.44216 |
1.2943 |
718.1 |
554.8 |
42.68 |
|
60.2 |
1.44238 |
1.2949 |
719.6 |
555.7 |
42.77 |
|
60.3 |
1.44261 |
1.2956 |
721.1 |
556.6 |
42.85 |
|
60.4 |
1.44284 |
1.2962 |
722.7 |
557.5 |
42.95 |
|
60.5 |
1.44306 |
1.2968 |
724.2 |
558.4 |
43.04 |
|
60.6 |
1.44329 |
1.2974 |
725.7 |
559.4 |
43.13 |
|
60.7 |
1.44352 |
1.2980 |
727.3 |
560.3 |
43.22 |
|
60.8 |
1.44375 |
1.2986 |
728.8 |
561.2 |
43.31 |
|
60.9 |
1.44398 |
1.2993 |
730.3 |
562.1 |
43.40 |
|
61.0 |
1.44420 |
1.2999 |
731.8 |
563.0 |
43.49 |
|
61.1 |
1.44443 |
1.3005 |
733.4 |
563.9 |
43.59 |
|
61.2 |
1.44466 |
1.3011 |
734.9 |
564.8 |
43.68 |
|
61.3 |
1.44489 |
1.3017 |
736.4 |
565.7 |
43.76 |
|
61.4 |
1.44512 |
1.3024 |
738.0 |
566.6 |
43.86 |
|
61.5 |
1.44535 |
1.3030 |
739.5 |
567.6 |
43.95 |
|
61.6 |
1.44558 |
1.3036 |
741.1 |
568.5 |
44.04 |
|
61.7 |
1.44581 |
1.3042 |
742.6 |
569.4 |
44.13 |
|
61.8 |
1.44604 |
1.3049 |
744.1 |
570.3 |
44.22 |
|
61.9 |
1.44627 |
1.3055 |
745.7 |
571.2 |
44.32 |
|
62.0 |
1.44650 |
1.3061 |
747.2 |
572.1 |
44.41 |
|
62.1 |
1.44673 |
1.3067 |
748.8 |
573.0 |
44.50 |
|
62.2 |
1.44696 |
1.3074 |
750.3 |
573.9 |
44.59 |
|
62.3 |
1.44719 |
1.3080 |
751.9 |
574.8 |
44.69 |
|
62.4 |
1.44742 |
1.3086 |
753.4 |
575.7 |
44.77 |
|
62.5 |
1.44765 |
1.3092 |
755.0 |
576.6 |
44.87 |
|
62.6 |
1.44788 |
1.3099 |
756.5 |
577.5 |
44.96 |
|
62.7 |
1.44811 |
1.3105 |
758.1 |
578.5 |
45.05 |
|
62.8 |
1.44834 |
1.3111 |
759.6 |
579.4 |
45.14 |
|
62.9 |
1.44858 |
1.3118 |
761.2 |
580.3 |
45.24 |
|
63.0 |
1.44881 |
1.3124 |
762.7 |
581.2 |
45.33 |
|
63.1 |
1.44904 |
1.3130 |
764.3 |
582.1 |
45.42 |
|
63.2 |
1.44927 |
1.3137 |
765.8 |
583.0 |
45.51 |
|
63.3 |
1.44950 |
1.3143 |
767.4 |
583.9 |
45.61 |
|
63.4 |
1.44974 |
1.3149 |
769.0 |
584.8 |
45.70 |
|
63.5 |
1.44997 |
1.3155 |
770.5 |
585.7 |
45.79 |
|
63.6 |
1.45020 |
1.3162 |
772.1 |
586.6 |
45.89 |
|
63.7 |
1.45043 |
1.3168 |
773.6 |
587.5 |
45.98 |
|
63.8 |
1.45067 |
1.3174 |
775.2 |
588.4 |
46.07 |
|
63.9 |
1.45090 |
1.3181 |
776.8 |
589.3 |
46.17 |
|
64.0 |
1.45113 |
1.3187 |
778.3 |
590.2 |
46.25 |
|
64.1 |
1.45137 |
1.3193 |
779.9 |
591.1 |
46.35 |
|
64.2 |
1.45160 |
1.3200 |
781.5 |
592.0 |
46.44 |
|
64.3 |
1.45184 |
1.3206 |
783.0 |
592.9 |
46.53 |
|
64.4 |
1.45207 |
1.3213 |
784.6 |
593.8 |
46.63 |
|
64.5 |
1.45230 |
1.3219 |
786.2 |
594.7 |
46.72 |
|
64.6 |
1.45254 |
1.3225 |
787.8 |
595.6 |
46.82 |
|
64.7 |
1.45277 |
1.3232 |
789.3 |
596.5 |
46.91 |
|
64.8 |
1.45301 |
1.3238 |
790.9 |
597.4 |
47.00 |
|
64.9 |
1.45324 |
1.3244 |
792.5 |
598.3 |
47.10 |
|
65.0 |
1.45348 |
1.3251 |
794.1 |
599.3 |
47.19 |
|
65.1 |
1.45371 |
1.3257 |
795.6 |
600.2 |
47.28 |
|
65.2 |
1.45395 |
1.3264 |
797.2 |
601.1 |
47.38 |
|
65.3 |
1.45418 |
1.3270 |
798.8 |
602.0 |
47.47 |
|
65.4 |
1.45442 |
1.3276 |
800.4 |
602.9 |
47.57 |
|
65.5 |
1.45466 |
1.3283 |
802.0 |
603.8 |
47.66 |
|
65.6 |
1.45489 |
1.3289 |
803.6 |
604.7 |
47.76 |
|
65.7 |
1.45513 |
1.3296 |
805.1 |
605.6 |
47.85 |
|
65.8 |
1.45537 |
1.3302 |
806.7 |
606.5 |
47.94 |
|
65.9 |
1.45560 |
1.3309 |
808.3 |
607.4 |
48.04 |
|
66.0 |
1.45584 |
1.3315 |
809.9 |
608.3 |
48.13 |
|
66.1 |
1.45608 |
1.3322 |
811.5 |
609.2 |
48.23 |
|
66.2 |
1.45631 |
1.3328 |
813.1 |
610.1 |
48.32 |
|
66.3 |
1.45655 |
1.3334 |
814.7 |
611.0 |
48.42 |
|
66.4 |
1.45679 |
1.3341 |
816.3 |
611.9 |
48.51 |
|
66.5 |
1.45703 |
1.3347 |
817.9 |
612.8 |
48.61 |
|
66.6 |
1.45726 |
1.3354 |
819.5 |
613.7 |
48.70 |
|
66.7 |
1.45750 |
1.3360 |
821.1 |
614.6 |
48.80 |
|
66.8 |
1.45774 |
1.3367 |
822.7 |
615.5 |
48.89 |
|
66.9 |
1.45798 |
1.3373 |
824.3 |
616.3 |
48.99 |
|
67.0 |
1.45822 |
1.3380 |
825.9 |
617.2 |
49.08 |
|
67.1 |
1.45846 |
1.3386 |
827.5 |
618.1 |
49.18 |
|
67.2 |
1.45870 |
1.3393 |
829.1 |
619.0 |
49.27 |
|
67.3 |
1.45893 |
1.3399 |
830.7 |
619.9 |
49.37 |
|
67.4 |
1.45917 |
1.3406 |
832.3 |
620.8 |
49.46 |
|
67.5 |
1.45941 |
1.3412 |
833.9 |
621.7 |
49.56 |
|
67.6 |
1.45965 |
1.3419 |
835.5 |
622.6 |
49.65 |
|
67.7 |
1.45989 |
1.3425 |
837.1 |
623.5 |
49.75 |
|
67.8 |
1.46013 |
1.3432 |
838.7 |
624.4 |
49.84 |
|
67.9 |
1.46037 |
1.3438 |
840.3 |
625.3 |
49.94 |
|
68.0 |
1.46061 |
1.3445 |
841.9 |
626.2 |
50.03 |
|
68.1 |
1.46085 |
1.3451 |
843.6 |
627.1 |
50.14 |
|
68.2 |
1.46109 |
1.3458 |
845.2 |
628.0 |
50.23 |
|
68.3 |
1.46134 |
1.3464 |
846.8 |
628.9 |
50.33 |
|
68.4 |
1.46158 |
1.3471 |
848.4 |
629.8 |
50.42 |
|
68.5 |
1.46182 |
1.3478 |
850.0 |
630.7 |
50.52 |
|
68.6 |
1.46206 |
1.3484 |
851.6 |
631.6 |
50.61 |
|
68.7 |
1.46230 |
1.3491 |
853.3 |
632.5 |
50.71 |
|
68.8 |
1.46254 |
1.3497 |
854.9 |
633.4 |
50.81 |
|
68.9 |
1.46278 |
1.3504 |
856.5 |
634.3 |
50.90 |
|
69.0 |
1.46303 |
1.3510 |
858.1 |
635.2 |
51.00 |
|
69.1 |
1.46327 |
1.3517 |
859.8 |
636.1 |
51.10 |
|
69.2 |
1.46351 |
1.3524 |
861.4 |
636.9 |
51.19 |
|
69.3 |
1.46375 |
1.3530 |
863.0 |
637.8 |
51.29 |
7. Expression of results
The results are expressed as a percentage by mass of saccharose m/m (degrees Brix) to two decimal based on the average of two determinations. In the case of the concentrated grape juice multiply the result by the dilution factor (F = 2.5).
8. Indicative characteristics of the method
A validation study was carried out for the purpose of assessing the suitability of the method for the matrix grape juice, taking into account linearity, limits of detection and quantification and the accuracy of the method. The latter parameter was determined by defining the levels of precision and trueness of the method.
8.1. Linearity of the method
Based on the results obtained from the linear regression analysis, the method proved to be linear within the ranges 10.0 °Bx to 69.2 °Bx shown in the Table III.
8.2. Limit of detection and limit of quantification
The detection limit (LD) and limit of quantification (LoQ) were calculated from a grape juice diluted and analyzed and is equal to 3 standard deviations for the LD and 10 standard deviations for the LoQ (Table III).
8.3. Precision of the method
The parameters taken into account were repeatability and reproducibility. Table III shows the values of these parameters. The repeatability was expressed as standard deviation of repeatability (Sr) of 7 measurements for the following °Brix concentrations: 15.9 °Bx; 69.2 °Bx.
The intermediate precision (IP) was expressed as the Standard Deviation (SIP) of measurements of the same grape juice sample, made by different operators.
Table III- Characteristics of the method
|
Linearity range (°Bx) |
Correlation coefficient (r2 ) |
LD (°Bx) |
LoQ (°Bx) |
Repeatability Standard Deviation
|
Intermediate Precision (IP) Standard Deviation
|
|
10.0 – 69.2 |
0.9999 |
0.5 |
1.7 |
0.03 °Bx |
0.04 °Bx |
9. Bibliography
- OIV. Compendium of International Methods of Analysis of Wines and Musts. Method OIV-MA-AS2-02.
- BS EN ISO 3696, Water for Analytical Laboratory Use — Specification and Test Methods, 1995.