Determination of the volatile acid content (Type II)
Method OIV-MA-VI-03 : R2000
Type II method
Determination of the volatile acid content in vinegars
(OENO 54/2000)
1. Definition
By convention, the volatile acidity of vinegar refers to the difference between the total acidity and the fixed acidity.
2. Principle
Calculation of difference between total acidity and fixed acidity, expressed in grams of acetic acid per L.
3. References
See the methods I (determination of total acidity content) and II (determination of the fixed acidity content).
4. Results
4.1. Calculation
Considering:
to be the total acidity content (expressed in grams of acetic acid per L of sample) and
to be the fixed acidity content (expressed in grams of acetic acid per L of sample).
The volatile acidity content expressed in grams of acetic acid per L of sample is given by:
 |
4.2. Presentation
The results expressed in grams of acetic acid per liter are given to the first decimal.
5. Interlaboratory validation (Hitos et al.2000)
Units: % (m/V)
| Sample | r | Sr | RSDr | R | SR | RSDR | RSDR
(Horwitz) |
Horrat
Index |
| 1 – 8.24% (m/v) | 0.0445 | 0.016 | 0.19 | 0.1632 | 0.058 | 0.71 | 2.91 | 0.24 |
| 2 – 11.17% (m/v) | 0.0438 | 0.016 | 0.14 | 0.1967 | 0.070 | 0.63 | 2.78 | 0.23 |
| 3 – 11.20% (m/v) | 0.0595 | 0.021 | 0.19 | 0.2076 | 0.074 | 0.66 | 2.78 | 0.24 |
| 4 – 11.94% (m/v) | 0.0473 | 0.017 | 0.14 | 0.1652 | 0.059 | 0.49 | 2.75 | 0.18 |
| 5 – 11.16% (m/v) | 0.0518 | 0.019 | 0.17 | 0.3577 | 0.0128 | 1.14 | 2.78 | 0.41 |
6. Bibliography
- Anonymous, 1993, Métodos Oficiales de Análisis, Tomo II, Ministério de Agricultura, Pesca y Alimentación, Madrid, Spain.
- AOAC, 1984, Official Methods of the Ass. Offic. Agric. Chem., 14th edit., Arlington USA.
- Hitos P., Pons A., Martin de la Hinojosa, I, Gomez R., Hernandez A. and Muñoz J., 2000. Validation of analysis methods for total, fixed and volatile acidity of non-volatile reducing substances, copper and zinc in wine vinegars, Green Sheet of OIV No. 115.